Communities associating algae and other microorganisms have a tremendous potential for environmental and biotechnological applications. The exploitation of these communities raises theoretical and practical challenges in mathematical modelling, optimization and control. In the Ctrl-AB project, we aim at developing new control methods to maximize productivity of a synthetic algal-bacterial community, and at demonstrating their effectiveness on state-of-the-art experimental platforms. We will develop coarse-grained models of the community dynamics, and leverage classical model-based and new data-driven methods for optimal controller design. We will exploit cutting-edge experimental technologies to develop molecular sensors and actuators enabling implementation of the control strategies. The Ctrl-AB project is carried out by a strong interdisciplinary consortium and will make novel contributions in control theory, mathematical biology and metabolic engineering.

Ctrl-AB is supported by the French National Research Agency (ANR), project ANR-20-CE45-0014

 

(A) Microalgae (green circles) producing lipids (orange spots) grow under suitable illumination, using vitamins synthesized by bacteria (purple rods). Vitamin synthesis is regulated by light at specific wavelengths thanks to optogenetics. Gaseous exchanges may also take place.
(B) Algae and bacteria are co-cultured in a continuous-flow bioreactor. The process is automated by a computer implementing control laws for maximizing lipid production.