La troisième réunion RNALands aura lieu à Paris entre le Mardi 24 et le Vendredi 27 Mai. Nous nous réunirons à l’Ecole Polytechnique sur un régime relâché, de façon à travailler en groupes plus restreints et avancer sur des problèmes précis 🙂
Mardi 24 Mai (Salle Thomas Flowers)
- Matin 10h30-12h30: Message de bienvenue & Dernières nouvelles (Yann+Andrea)
- Après-midi 13h30-17h30: Point sur le clustering de structures d’ARN et collecte de données
Mercredi 25 Mai (Salle Thomas Flowers)
- Matin 10h30-13h : Progrès sur les méthodes d’échantillonnage/RNAXplorer
- Après-midi 14h-??? : Départ pour le musée Montmartre + Dinner à Paris
Jeudi 26 Mai (Salle Marcel-Paul Schützenberger)
- Matin 10h30-13h: Analyse des paysages de repliement
- Après-midi 14h30-15h30 : Exposé de Hervé Isambert (Institut Curie)
RNA swiches and nanostructures under transcriptional control
We explored, using both computational and experimental approaches, the properties of small regulatory circuits primary based on RNAs and their interactions. In particular, we used synthetic biology approaches coupled to advanced RNA simulations (kinefold.curie.fr) to design efficient RNA-based regulatory modules inspired from bacterial RNA switches. These modules control RNA transcription « on the fly » through RNA-RNA antisense interactions and underline our ability to predictably encode and regulate the folding of nascent RNA in the course of transcription.We also discovered that DsrA, a small bacterial regulatory RNA, self-assembles, like many proteins do, to form long filaments as well as extended nanostructures. This hierarchy of ncRNA supramolecular structures forming at room temperature in the course of transcription relies on a self-assembly topology different from the assembly of DNA origami. The resulting RNA nanostructures store internal mechanical constraints which can be subsequently relaxed, resulting in cooperative structural switching of the RNA nanostructure.These findings further extend the already great versatility of natural RNA functions in cells, while opening up new avenues for the design of synthetic RNA/DNA nanostructures with addressable structural switching properties
Vendredi 27 Mai (Salle Marcel-Paul Schützenberger)
- Matin 10h30-12h30 : Collaboration en petits groupes
- Déjeuner : Conclusions, organisation des journées suivantes à Vienne, école d’été/hiver, des prochaines séjours de recherches …
